Synthesis of Photo Crosslinked and pH Sensitive Polymersomes and Applications in Synthetic Biology

As an inspiration from nature, polymeric vesicles can be formed from amphiphilic block-copolymers. These vesicles are called polymersomes and have applications in drug delivery and as nanoreactors. Within this thesis, photo cross-linked and pH sensitive polymersomes were synthesized,... Ausführliche Beschreibung

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Weitere Verfasser: [advisor] ; [referee]
Format: Elektronische Hochschulschrift
veröffentlicht: Dresden : Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2013.
Schlagworte:
RVK-Notation: VK 8007 Dissertationen, Habilitationsschriften, wertvollere und umfangreichere Sonderdrucke
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041 |a eng 
037 |n urn:nbn:de:bsz:14-qucosa-109715 
100 |a Jens Gaitzsch  |e author 
700 |a Brigitte Voit  |e advisor 
700 |a Brigitte Voit  |e referee 
700 |a Petra Schwille  |e referee 
245 |a Synthesis of Photo Crosslinked and pH Sensitive Polymersomes and Applications in Synthetic Biology 
260 |a Dresden :  |b Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden,  |c 2013.  |9 (issued 2013-04-10) 
520 3 |a As an inspiration from nature, polymeric vesicles can be formed from amphiphilic block-copolymers. These vesicles are called polymersomes and have applications in drug delivery and as nanoreactors. Within this thesis, photo cross-linked and pH sensitive polymersomes were synthesized, characterized and applied on cells as well as bionanoreactors. The stability due to the crosslinking yielded polymersomes which show a distinct and reproducible swelling upon repeated pH changes. If the non cross-linked vesicles were exposed to a plasma-cleaned surface, they formed a tethered singly and multiple bilayers. Upon studying these membranes, they turned out to harden upon crosslinking and showed a completely non-fluid behaviour. Additionaly, the polymersome-cell interactions were studied and yielded a high influence of the crosslinking conditions on cellular toxicity. If crosslinked for a long time in a phosphate-free enviroment, the polymersomes proved to be least toxic. Finally, an enzyme was incorporated into the polymersomes to create bionanoreactors. Due to the pH sensitivity and swelling, the vesicles created yielded a pH controlled nanoreactor with enzymatic activity and a swollen, e.g. acidic, state only. 
500 |a doctoralThesis 
856 4 0 |u http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-109715 
856 4 1 |u http://www.qucosa.de/fileadmin/data/qucosa/documents/10971/PhDThesisJensGaitzsch.pdf 
650 4 |a Synthetische Biologie 
650 4 |a Polymersome 
650 4 |a Blockcopolymere 
650 4 |a Selbstassoziation 
650 4 |a Molekül-Freisetzung 
650 4 |a Nanoreaktor 
650 4 |a Synthetic Biology 
650 4 |a Polymersomes 
650 4 |a Block-Copolymers 
650 4 |a Self-Assembly 
650 4 |a Drug-Delivery 
650 4 |a Nanorector 
082 0 |a 540 
084 |2 rvk  |a VK 8007  |9 V  |9 VK  |9 VK 5000 - VK 8809  |9 VK 8000 - VK 8009  |9 VK 8007 
936 r v |a VK 8007  |b Dissertationen, Habilitationsschriften, wertvollere und umfangreichere Sonderdrucke  |k Chemie und Pharmazie  |k Organische Chemie  |k Organische Chemie allgemein  |k Polymere  |k Dissertationen, Habilitationsschriften, wertvollere und umfangreichere Sonderdrucke 
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852 |a DE-D117  |z 2017-04-26T13:59:46Z 
852 |a FID-BBI-DE-23  |z 2019-01-21T06:39:49Z 
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852 |a DE-D13  |z 2017-04-26T13:59:46Z 
980 |a urn:nbn:de:bsz:14-qucosa-109715  |b 22  |c 201704261642 
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